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Gently resuspend by pipetting the rhLaminin-521 dilution up and down. Note: This results in a working concentration of 2.5 µg/mL (i.e., a 1:40 dilution). 3. Add 2 mL of the diluted rhLaminin-521 solution to each well of a Biolaminin 521 LN (LN521) is the natural laminin for pluripotent stem cells and therefore reliably facilitates self-renewal of human ES and iPS cells in a chemically defined, feeder-free and animal component-free stem cell culture system. For single-cell culture of hPSCs on another Biolaminin matrix than 521 or 511, ROCKi must be added. Alternatively, mix in one part of 521 with the other Biolaminin isoform (1:3 mix) and use for coating.

Årsredovisning 2017 - Finansinspektionen

Note: This results in a working concentration of 2.5 µg/mL (i.e., a 1:40 dilution). 3. Add 2 mL of the diluted rhLaminin-521 solution to each well of a Biolaminin 521 LN (LN521) is the natural laminin for pluripotent stem cells and therefore reliably facilitates self-renewal of human ES and iPS cells in a chemically defined, feeder-free and animal component-free stem cell culture system. For single-cell culture of hPSCs on another Biolaminin matrix than 521 or 511, ROCKi must be added. Alternatively, mix in one part of 521 with the other Biolaminin isoform (1:3 mix) and use for coating.

1 May 2013 BioLamina reports that its laminins recreate specific cell niches in a cell the blastocyst where stem cells secrete and grow on laminin-521 and 10 Mar 2021 Laminin-521, BioLamina. TrypanBlue + Burker chamber or any other counting device.